category
Nature Protocols
date
Feb 4, 2026
slug
status
Published
summary
开发了高通量原生质体测定方法，实现了CRISPRi基因电路在多种植物中的快速验证，提供了可编程基因电路设计的标准化流程。
tags
基因编辑
合成生物学
type
Post
📄 原文题目
Designing and testing CRISPRi-based synthetic gene circuits in plants
🔗 原文链接
💡 AI 核心解读
开发了高通量原生质体测定方法，实现了CRISPRi基因电路在多种植物中的快速验证，提供了可编程基因电路设计的标准化流程。
📝 英文原版摘要
<p>Nature Protocols, Published online: 04 February 2026; <a href="https://www.nature.com/articles/s41596-025-01312-y">doi:10.1038/s41596-025-01312-y</a></p>This protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant species using a high-throughput protoplast-based assay.