category
bioRxiv
date
Mar 19, 2026
slug
status
Published
summary
首次在Zymomonas mobilis中系统表征了NahR-PsalTTC、VanRAM-PvanCC、CinRAM-Pcin和LuxR-PluxB等七种化学诱导启动子,并验证了基于pZMO7穿梭载体的高效表达系统,其中VanRAM-PvanCC和CinRAM-Pcin表现出特别广阔的应用前景。
tags
合成生物学
type
Post

📄 原文题目

Seven inducible promoters for Zymomonas mobilis

🔗 原文链接

💡 AI 核心解读

首次在Zymomonas mobilis中系统表征了NahR-PsalTTC、VanRAM-PvanCC、CinRAM-Pcin和LuxR-PluxB等七种化学诱导启动子,并验证了基于pZMO7穿梭载体的高效表达系统,其中VanRAM-PvanCC和CinRAM-Pcin表现出特别广阔的应用前景。

📝 英文原版摘要

Zymomonas mobilis is an ethanologenic Alphaproteobacterium with many interesting characteristics for fundamental research and applied microbial engineering. Although genetic engineering has been established for Z. mobilis since the 1980s, a rich set of inducible transcriptional regulators is still unavailable. In this work, seven different chemically inducible promoters have been systematically tested for their functionality in Z. mobilis. In particular, for the first time, NahR-PsalTTC, VanRAM-PvanCC, CinRAM-Pcin and LuxR-PluxB have been characterized in Z. mobilis, alongside the commonly used regulator-promoter pairs TetR-Ptet and LacI-PlacT7A1_O3O4, and the less commonly used XylS-Pm. All promoters investigated in this work are compatible with the Golden Gate modular cloning framework Zymo-Parts. Characterization was carried out with a shuttle vector backbone based on pZMO7, which has so far been rarely used for applications in Z. mobilis but seems to be completely stable without selection and generates high and uniform levels of expression. From the experimental results presented, it can be concluded that VanRAM-PvanCC and CinRAM-Pcin are particularly promising for broad use in the Z. mobilis community.
SNMF:面向空间转录组学的超快速、空间感知解卷积方法古代马的体型、牙科病理学及母系遗传多样性:波罗的海东部地区和俄罗斯西部的研究
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