category
bioRxiv
date
Mar 10, 2026
slug
status
Published
summary
创新性地实现了无需基因强制表达、细胞分选、啮齿动物共培养或脑内移植的高效体外生成方法;通过单细胞转录组分析验证了神经元的体内真实性;在50天内完成生成过程,显著缩短了传统方法的耗时。
tags
单细胞测序
type
Post

📄 原文题目

Rapid and reproducible in vitro generation of human parvalbumin-expressing cortical interneurons

🔗 原文链接

💡 AI 核心解读

创新性地实现了无需基因强制表达、细胞分选、啮齿动物共培养或脑内移植的高效体外生成方法;通过单细胞转录组分析验证了神经元的体内真实性;在50天内完成生成过程,显著缩短了传统方法的耗时。

📝 英文原版摘要

Parvalbumin-expressing cortical interneurons play a critical role in maintaining the balance between excitatory and inhibitory signalling and are essential for cognition, with dysfunction implicated in numerous brain disorders. Although human pluripotent stem cells have enabled the generation of diverse human neuronal types in vitro, including cortical interneurons, parvalbumin-expressing interneurons - unlike somatostatin-expressing interneurons - remain difficult to generate reliably and consistently. Here, we demonstrate the efficient and reproducible generation of parvalbumin-expressing cortical interneurons in vitro within 50 days of differentiation. Parvalbumin mRNA and protein were detected without forced gene expression, cell sorting, rodent co-culture or intracerebral transplantation, approaches commonly required by previous protocols. Single-cell transcriptomic analyses validated neuronal identity and authenticity, revealing enrichment for gene expression signatures of parvalbumin-expressing cortical interneurons in vivo. Together, these findings establish a robust method that facilitates interneuron research by enabling the reliable generation of authentic human parvalbumin-expressing cortical interneurons within a short time frame.
心肌细胞固有的SLC25A1调节心脏分化和线粒体功能灵长类动物X连锁扩增基因的普遍正选择
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