结核分枝杆菌MutT4/RppH是一种RNA焦磷酸酶，可形成凝聚体样结构并影响mRNA降解

📄 原文题目

Mycobacterium tuberculosis MutT4/RppH is an RNA pyrophosphohydrolase that forms condensate-like bodies and impacts mRNA degradation

🔗 原文链接

https://academic.oup.com/nar/article/doi/10.1093/nar/gkag200/8521936?rss=1

💡 AI 核心解读

📝 英文原版摘要

<div class="boxTitle">Abstract</div>Bacterial adaptation to stress involves changes in transcription and messenger RNA (mRNA) degradation. In Escherichia coli, the Nudix hydrolase RppH initiates mRNA degradation by removing pyrophosphate from mRNA 5′-ends, converting 5′-triphosphates to 5′-monophosphates. We aimed to identify the RppH homolog in the globally important pathogen Mycobacterium tuberculosis (Mtb). We identified the protein encoded by Rv3908, previously annotated as a nucleotide pool cleanser mutT4, as the predominant mycobacterial RppH. Deletion of rppHMtb increased the relative abundance of 5′-triphosphates on myriad mRNAs across the transcriptome. Purified RppHMtb converted mRNA 5′-triphosphates into monophosphates, and stimulated degradation by RNase E and RNase J in vitro to varying extents. Surprisingly, deletion of rppHMtb had mixed impacts on mRNA degradation in vivo, suggesting that it may not sensitize most transcripts to degradation. RppHMtb has intrinsically disordered regions (IDRs), which often participate in biomolecular condensate formation. Microscopy showed that RppHMtb forms condensate-like bodies that localize with RNases and dissociate upon addition of rifampicin. The N-terminal IDR is sufficient for condensate-like body formation. Deletion of rppHMtb leads to higher outer membrane permeability and resistance to oxidative stress. We conclude that MutT4

is the mycobacterial RppH, assembling in condensate-like bodies with RNases but having unexpectedly complex impacts on mRNA degradation rates.