category
Nature Communications
date
Mar 3, 2026
slug
status
Published
summary
开发了基于CRISPR碱基编辑和高通量测序的端到端框架，首次实现单核苷酸分辨率的调控元件定位，发现增强子突变与CD19表达调控及CAR-T疗法耐药性的关联。
tags
测序技术
基因编辑
type
Post
📄 原文题目
Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing
🔗 原文链接
💡 AI 核心解读
开发了基于CRISPR碱基编辑和高通量测序的端到端框架，首次实现单核苷酸分辨率的调控元件定位，发现增强子突变与CD19表达调控及CAR-T疗法耐药性的关联。
📝 英文原版摘要
<p>Nature Communications, Published online: 03 March 2026; <a href="https://www.nature.com/articles/s41467-026-69918-8">doi:10.1038/s41467-026-69918-8</a></p>Regulatory DNA screens often lack nucleotide-level resolution. Here, authors present an end-to-end CRISPR base-editing and sequencing framework that maps regulatory variants at single-nucleotide resolution, revealing enhancer mutations that alter CD19 expression and enable CAR-T therapy resistance.
- 作者:NotionNext
- 链接:https://tangly1024.com/article/31848bd6-1f96-812d-bfa2-fbb6cf1362d3
- 声明:本文采用 CC BY-NC-SA 4.0 许可协议，转载请注明出处。
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