<p>Nature Communications, Published online: 10 March 2026; <a href="https://www.nature.com/articles/s41467-026-68596-w">doi:10.1038/s41467-026-68596-w</a></p>Single-cell transcriptome profiling has been reported for human thymi, but high-resolution spatial information is still lacking. Here the authors use Stereo-seq to report spatial transcriptomic and proteomic information from human fetal and pediatric thymi to provide a useful resource, and to define transcription factor networks regulating rare thymic cell types.